Dynamics of the microorganisms’ number in the soil mass of podzolized chernozem after treatment with fungicides of various origins
DOI:
https://doi.org/10.31073/acss95-05Keywords:
soil; fungicides; soil micromycetes; microbial communities; functional state of soil microbial coenosisAbstract
In a laboratory model experiment, the dynamics of the number of bacteria and micromycetes in the soil mass of podzolized chernozem after a one-time treatment of its surface with fungicides of various origins investigated. Chemical fungicides based on the active substances carbendazim, tebuconazole and azoxystrobin, and biological fungicides based on the antagonistic fungi Trichoderma viride (lignorum) and Chaetomium cohliodes were used. The soil mass for the experiment was taken from the arable layer of the soil on the site without fertilizers in the field experiment of the Department of Agrochemistry NSC "Institute for Soil Science and Agrochemistry Research named after O. N. Sokolovsky" (NSC "ISSAR") on the territory of SE "EF Hrakivske" (Noviy Korotych village, Kharkiv district, Kharkiv region). During 110 days of composting at a constant temperature, changes in the state of microbial communities, in particular soil micromycetes, and the speed and completeness of their recovery compared to soil not treated with fungicides evaluated. The state of the microflora was monitored four times during the study period according to the parameters of the number of microorganisms belonging to various ecological-trophic and taxonomic groups, the total biological index (TBI) and indicators reflecting the functional state of microbial coenoses (oligotrophy and mineralization indices, the coefficient of microbial transformation of soil organic matter (MTSOM). On the fifth day after treatment with fungicides, the number of bacteria and micromycetes in the samples of the treated soil mass was slightly higher higher than in the control samples, and on the twentieth day, on the contrary, it was significantly lower. The number of bacteria was lower by 20-46 %, micromycetes by 16-34 % compared to the control. On the fortieth day, a partial restoration of the number of bacteria to the level of the untreated control soil observed, and the number of micromycetes remained lower by 9-36 % compared to the control in the case of chemical fungicides. When using biological fungicides, the number of micromycetes approached the values in control soil and accounted for 84-94 % of them. On the one hundred and tenth day, the number of microflora in the soil treated with chemical fungicides according to TBI was 79-89 % of the control values, and in the soil treated with biofungicides it was 102-104 %. The number of micromycetes decreased to a lesser extent and recovered most completely and quickly in the case of the application of the biological fungicide Hetomic. Given the important role of soil micromycetes in the functioning of soils, a decrease in their number due to the accumulation of residual amounts of chemical fungicides in the soil can have a negative impact on many soil-biological processes, in particular the decomposition of plant residues and further transformation of organic matter, as well as on the phytosanitary state of the soil. Therefore, in order to avoid the possible consequences of their excessive or repeated application and the associated threat of soil contamination, it is necessary to carry out microbiological monitoring of soils in areas with a high fungicide load.
References
References
Aktar, W., Sengupta, D., & Chowdhury, A. (2009). Impact of Pesticides use in agriculture their benefits and hazards. Interdisciplinary toxicology, 2. Р. 1-12.
Vozhegova, R. A., Zayecz, S. O., & Fundyrat, K. S. (2021). Effectiveness of biological and chemical fungicides in the fight against pathogens of fungal diseases on winter barley crops under irrigation conditions. Visnyk agrarnoyi nauky, 11, P. 67-74. [in Ukrainian].
Muyesaier, T., Huada, D. R., & Wang, L. (2021). Agriculture development, pesticide application and its іmpact on the environment. Environmental Research and Public Health, 3. Р. 1112. https://doi.org/10.3390/ijerph18031112
Al-Ani, M., Hmoshi, R., Kanaan, I., & Thanoon, A. (2019). Effect of pesticides on soil microorganisms. Journal of Physics: Conference Series, 1. https://doi.org/10.1088/1742-6596/1294/7/072007
Bublik, L. I., & Kruk, I. V. (2008). Methods of monitoring soil pesticide contamination of agroecocenoses. Zakhyst i karantyn roslyn, 54, 87-99. [in Ukrainian].
Andrea, M. M., Peres, B. B., Luchini, L. C., & Pettinelli, A. J. (2000). Impact of long-term pesticide application on some soil biological parameters. Journal of Environmental Science and Health, 35. Р. 297-307. https://doi.org/10,1080/03601230009373271
Kalia, A., & Gosal, S. K. (2011). Effect of pesticide application on soil microorganisms. Archives of Agronomy abd Soil Science, 35. Р. 669-596. https://doi.org/10.1080/03650341003787582
Baxter, J., & Cummings, S. P. (2008). The degradation of the herbicide bromoxynil and its impact on bacterial diversity in a top soil. Journal of applied microbiology, 4. Р. 1605-1616. https://doi.org/10.1111/j.1365-2672.2007.03709.x
Niewiadomska, A. (2004). Effect of carbendazim, imazetapir and thiramon nitrogenase activity, the number of microorganisms in soil and yield of red clover (Trifolium pretense L.). Polish Journal of Environmental Studies, 13. P. 403-410
Myshustyn, E. N., & Emcev, V. T. (1978). Мicrobiology. Moskva: Vуsshaya shkola, Р. 350. [in Russian].
Bridge, P., & Spooner, B. (2001). Soil fungi: diversity and detection. Plant and Soil, 232. Р. 147-154
Dighton, J. (2003). Fungi in ecology рrocesses. New York: Marcel Dekker, Р. 443.
Frac, M., Hannula, S.E., Belka, M., & Jedryczka, M. (2018). Fungal biodiversity and their role in soil health. Frontiers in microbiology, 9. Р. 56-65.
Sun, X., Zhu, L., Wang, J., Wang, J., Su, B., Du, Z., & Pengpeng, G. (2017). Effects of endosulfan on the populations of cultivable microorganisms and the diversity of bacterial community structure in brunisolic soil. Water, Air, & Soil Pollution., 228, Р. 1-11.
Boyardyn, A. N., Prudnykova, S. V., & Fylypenko, M. L. (2012). Biodegradation of polyhydroxyalkanoates by soil microbiocenoses of various structures and identification of destructive microorganisms. Prykladnaya byokhіmyya і mykrobіologіya, 1, 35-44. [in Russian].
Ingram, C. W., Coyne, M., & Williams, D. W. (2005). Effects of commercial diazinon and imidacloprid on microbial urease activity in soil. Journal of Environmental Quality, 5. Р. 1573-1580. https://doi.org/10.2134/jeq2004.0433
Littlefield-Wyer, J. G., Brooks, P., & Katouli, M. (2008). Application of biochemical fingerprinting and fatty acid methyl ester profiling to assess the effect of the pesticide Atradex on aquatic microbial communitie. Environmental Pollution, 13. Р. 393-400. https://doi.org/10.1016/j.envpol.2007.08.016
Vazquez-Blanco, R., Arias-Estevez, M., Baath, E., & Fernandez-Calvino, D. (2020). Comparison of Cu salts and commercial Cu based fungicides on toxicity towards microorganisms in soil. Environmental Pollution, 257. Р. 113585. https://doi.org/10.1016/j.envpol.2019.113585
Sardar, D. K., & Kole, R. K. (2005). Metabolism of chlorpyrifos in relation to its effect on the availability of some plant nutrients in soil. Chemosphere, 9. Р. 1273-1280. https://doi.org/10.1016/j.chemosphere.2005.03.078
Bacmaga, M. W., Wyszkowska, J., & Kucharski, J. (2020). Response of soil microorganisms and enzymes to the foliar application of Helicur 250 EW fungicide on Horderum vulgare. Chemosphere, 242. Р. 125163 https://doi.org/10.1016/j.chemosphere.2019.125163
Wang, C., Wang, F., Zhang, Q., & Liang, W. (2016) Individual and combined effects of tebuconazole and carbendazim on soil microbial activity. European Journal of Soil Biology, 72. Р. 6-13.
Soil quality. Determination of the number of microorganisms in the soil by the method of sowing on a solid (agarized) nutrient medium: DSTU 7847:2015 (National Standard of Ukraine) [Valid from 01.07.2016].
Arystovskaya, T. V., & Xudyakova, Yu. A. (1977). Methods of studying soil microflora and its vital activity. Metodу stacyonarnogo іzuchenyya pochv. Moskva: Nauka, P. 141-286. [in Russian].
Myshustyn, E. N. (1975). Associations of soil microorganisms. Moskva: Nauka, Р. 24. [in Russian].
Muha, V. D. (1980). About indicators reflecting the intensity and direction of soil processes. Sb. nauch. tr. XSXY, 273, P. 13-16. [in Russian].
Naydyonova, O. E., & Baliuk, S. A. (2014). Biological degradation of Chernozems under irrigation. Eurasian Journal of Soil Science, Vol. 3. Issue 4. P. 267–273. https://doi.org/10.18393/ejss.87170
Singh, S., Singh, N., Kumar, V., & Datta, S. (2016). Toxicity, monitoring and biodegradation of the fungicide carbendazim. Environmental Chemistry Letters, 14. Р. 317-329. https://doi.org/10.1007/s10311-016-0566-2
Wang X., Song M., Gao C., Dong B., Zhang Q., & Fang H., Yu Y. (2009). Carbendazim induces a temporary change in soil bacterial community structure. Journal of Environmental Sciences, Vol. 21, Issue 12. 2009, P. 1679–1683. https://doi.org/10.1016/S1001-0742(08)62473-0
Wang, Y. S., Huang, Y. J., Chen, W.C., & Yen, J. H. (2009). Effect of carbendazim and pencycuron on soil bacterial community. Journal of Hazardous Materials, Vol. 172, Issue 1. P. 84–91. https://doi.org/10.1016/j.jhazmat.2009.06.142
Yan, H., Wang, D., Dong B., Tang F., Wang B., Fang H., & Yu Y. (2011). Dissipation of carbendazim and chloramphenicol alone and in combination and their effects on soil fungal:bacterial ratios and soil enzyme activities. Chemosphere, Vol. 84, Issue 5, P. 634–641. https://doi.org/10.1016/j.chemosphere.2011.03.038
Guilong, Ma, Xinxin, G., Jie, N., Tingting, Z., Xiaobao, X., & Qi, C. (2021). Fungicides alter the distribution and diversity of bacterial and fungal communities in ginseng fields. Bioengineered, 1 (12). Р. 8043–8056. https://doi.org/10.1080/21655979.2021.1982277
Küçük, С., Yeşilorman, D., & Cevheri, С. (2016). Effect of some fungicides on soil biological activities in laboratory conditions. Adıyaman University Journal of Science, 6 (2), P. 187-201.
Roman, D. L., Voiculescu, D. I., Filip, M., Ostafe, V., & Isvoran, A. (2021). Effects of Triazole Fungicides on Soil Microbiota and on the Activities of Enzymes Found in Soil: A Review. Agriculture, 9. Р. 893-911. https://doi.org/10.3390/agriculture1
Muñoz-Leoz, B., Ruiz-Romera, E., Antigüedad, I., & Garbisu, C. (2011). Tebuconazole application decreases soil microbial biomass and activity. Soil Biology and Biochemistry, 10 (43). Р. 2176 – 2183. https://doi.org/10.1016/j.soilbio.2011.07.001
Bacmaga, M., Kucharski, J., & Wyszkowska, J. (2015). Microbial and enzymatic activity of soil contaminated with azoxystrobin. Environmental Monitoring and Assesment, 10. Р. 615-629. https://doi.org/10.1007/s10661-015-4827-5
Diedhiou, P. M., Oerke, E. C. & Dehne, H. W. (2004). Effects of the strobilurin fungicides azoxystrobin and kresoxim-methyl on arbuscular mycorrhiza. Journal of Plant Diseases and Protection, Vol. 111. No. 6. Pp. 545– 556. https://www.jstor.org/stable/43215611
Buck, J. W., & Burpee, L. L. (2002) The effects of fungicides on the phylloplane yeast populations of creeping bentgrass. Canadian Journal of Microbiology, 6(48). P. 522 – 529.
Adetutu, E. M., Ball, A. S., & Osborn, A. M. (2008). Azoxystrobin and soil interactions: degradation and impact on soil bacterial and fungal communities. Journal of Applied Microbiology, 105. Р. 1777-1790. https://doi.org/10.1111/j.1365-2672.2008.03948.x
Bertelsen, J. R., De Neergaard, E., & Smedegaard-Petersen, V. (2001). Fungicidal effects of azoxystrobin and epoxiconazole on phyllosphere fungi, senescence and yield of winter wheat. Plant Pathology, 50. Р. 190-205.
Wang X., Lu Z., Miller H., Liu J., Hou Z., Liang S., Zhao X., Zhang H., Borch T. (2020). Fungicide azoxystrobin induced changes on the soil microbiome. Applied Soil Ecology, Vol. 145. 103343. https://doi.org/10.1016/j.apsoil.2019.08.005
Becker, P., Esker, P., & Umaña, G. (2021). Incorporation of microorganisms to reduce chemical fungicide usage in black sigatoka control programs in Costa Rica by use of biological fungicides. Crop Protection, Vol. 146. 105657. https://doi.org/10.1016/j.cropro.2021.105657
Hetman, Ya. V., & Naidyonova, O. E. (2021). Dynamics of the number of micromycetes in podzolized chernozem with the use of different fungicides. Biological processes of optimizing the production process of cultivated plants: materials of the All-Ukrainian scientific and practical online conference dedicated to the 60th anniversary of ISMAV NAAS (Chernihiv, October 26-27, 2021), National Academy of Agrarian Sciences of Ukraine, Institute of Agricultural Microbiology and Agro-Industrial Production, Society of microbiologists of Ukraine named after S.M. Vinohradskyi. Chernihiv: O.V. Brahynets publisher. P. 57–59. https://ismav.com.ua/wp-content/uploads/2021/12/%D0%A2%D0%B5%D0%B7%D0%B8-2021_%D0%905.pdf [in Ukrainian].
Downloads
Published
Issue
Section
License
This work is distributed under the Creative Commons Attribution-NonCommercial 4.0 International License.
