Abstract.
The issue of increasing resistance to extreme factors is becoming increasingly important, the relevance of the search for methods and ways to reduce the impact of abiotic stresses (droughts, high temperatures, frosts) on the implementation of the adaptive potential of agricultural crops is growing. For some unfavorable factors, the antistress activity of phytohormones, microelements and micronutrients was investigated, however, the greatest efficiency can be achieved through the combination of the necessary nutrients, hormones and growth regulators in an optimal rate. The aim of the work was establishing the effectiveness of the combined application of fertilizers, stress protectors and plant growth regulators to mitigate the effects of abiotic stresses. One of the tasks was to determine the optimal doses and methods of applying stress protectors. In a laboratory experiment, the effect on seed germination (by indications: sprouting, energy friendliness and germination rate), identified a set of components for complexing in the integrated products. As a result, we created two complex stress-protective fertilizers (sets of separate preparations) to mitigate the effects of abiotic stresses. The first one - SPs, is intended for seed treatment before sowing; it includes a complex of trace elements, sodium tripolyphosphate, phytohormones and silicon, as well as a naturally occurring polysaccharide lipogenic composition. The second preparation - SPf, intended for foliar fertilizing plants, contains the same components, as well as a complex of L-configuration amino acids and macronutrients in mineral form. The effect of complex preparations against the background of mineral fertilization (N30P30K30) was investigated in two small-plot experiments on barley crops. The greatest effect on the barley yield was recorded when combining mineral fertilizers with the use of pre-sowing seed treatment with SPs at a rate of 1 l/t and a one-time foliar fertilizing plants during the growing season with SPf at a rate of 1.0 l/ha or 0.5 l/ha twice during vegetation.
References
1. Waraich E.A, Ahmad R., Ullah S., Ashraf M.Ya., Ehsanullah. 2011. Role of mineral nutrition in alleviation of drought stress in plants. Australian Journal of Crop Science. 5(6). P. 764-777 URL: https://www.researchgate.net/ publication/236119726_Role_of_mineral_nutrition_in_alleviation_of_drought_stress_in_plants.
2. Zhuk O.I. 2011. Formation of adaptive response of plants to water deficiency. Physiology and biochemistry of plant cultures. 43(1). P. 26-37. URL: http://dspace.nbuv.gov.ua/bitstream/handle/123456789/66347/03-Zhuk.pdf?sequence=1. (Ukr.).
3. Kudoyarova G.R., Dodd I.C., Veselov D.S., Rothwell S.A., Veselov S.Yu. 2015. Common and specific responses to availability of mineral nutrients and water. J. Exp Bot. Apr. 66(8). P. 2133–2144. DOI: https://doi.org/10.1093/jxb/erv017.
4. IAEA (International Atomic Energy Agency). Nutrient and water management practices for increasing crop production in rainfed arid/semi-arid areas. IAEATECDOC-1468. IAEA, Vienna, Austria. 2005. 120 p. URL: https://www-pub.iaea.org/MTCD/Publications/PDF/te_1468_web.pdf.
5. Selye H.A. 1936. Syndrome produced by diverse nocous agent. Nature. 138. P. 32. DOI: https://doi.org/10.1038/138032a0
6. Kolupaev Yu.E., Karpets Yu.V. 2010. Formation of adaptive reactions of plants to the action of abiotic stressors. Kuiv: Osnova. 352 p. (Rus.).
7. Kolupaev Yu.E., Gorelova E.I., Yastreb T.O. 2018. Mechanisms of plant adaptation to hypothermia: the role of the antioxidant system. Visnyk of KhNAU. Biology. №1. P. 6-33. (Rus.).
8. Pyatygin S.S. 2008. Stress in plants: a physiological approach. Journal. general biology. 69. № 4. P. 294–295. (Rus.).
9. Vedenichova N.P., Kosakivska I.V. 2017. Cytokinins as regulators of plant ontogenesis under different growth conditions. Kyiv: Nash format, 200 p. (Ukr.).
10. Kushnir O.V., Kuryata V.G. 2018. Physiological bases of application of phytohormones and antihiberelin drugs in plant growing. Modern problems of biological science and methods of its teaching in higher education institutions. P. 244–261. (Ukr.).
11. Kefeli V.I., Vlasov P.V., Prusakova L.D. 1990. Natural and synthetic regulators of plant ontogenesis. T. 7. Moscow: series of plant physiology, 1990.157 p. (Rus.).
12. Polyanchikov S., Kapitanska O. 2018. The market of biostimulants: prospects for development in Ukraine. URL: http://infoindustria.com.ua/rinok-biostimulyatoriv-perspektivi-dlya-rozvitku-v-ukrayini/ (Ukr.).
13. Popov A.I. 2004. Humic substances, properties, structure, education. St. Petersburg. 248 p. (Rus.).
14. Sergienko V. 2018. Growth regulatory and protective effect of humic substances. Agribusiness Today. URL: https://tdnasinnya.com/uk/statti/inshi/140-ristregulyuyuchij-ta-zaxisnij-efekt-guminovix-rechovin (Ukr.).
15. Van Oosten M.J., Pepe O., De Pascale S., Silletti S., Maggio A. 2017. The role of biostimulants and bioeffectors as alleviators of abiotic stress in crop plants. Chem. Biol. Technol. Agric. 4: 5. DOI: https://doi.org/10.1186/s40538-017-0089-5
16. Vikhreva V.A., Lebedeva T.B., Nadezhkina E.V. 2011. The use of anti-stress drugs for Рherbicidal treatment of spring barley crops. Agrochemistry. № 5. Р. 46-53. URL: http://naukarus.com/primenenie-antistressovyh-preparatov-pri-gerbitsidnoy-obrabotke-posevov-yarovogo-yachmenya (Rus.).
17. Saporta R., Bou C., Frías V., Mulet J.M. 2019. A Method for a Fast Evaluation of the Biostimulant Potential of Different Natural Extracts for Promoting Growth or Tolerance against Abiotic Stress. Agronomy. 9. 143. DOI: https://doi.org/10.3390/agronomy9030143.
18. Osipova L.V., Kurnosova T.L., Bykovskaya I.A. 2016. Increasing the adaptive potential of spring barley (Hordeum Vulgare l.) under the action of abiotic stress. Problems of agrochemistry and ecology. 3. P. 48-51. URL. http://agroproblem.soil.msu.ru/index.php/content/10-uncategorised/nomer/statya/86-2016-3-48 (Rus.).
19. Kolupaev Yu.E., Yastreb T.O., Lugovaya A.A. 2016. The role of jasmonates in plant adaptation to abiotic stress. Plant physiology and genetics. 48(2). P. 95-111. URL: http://www.irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe?I21DBN=LINK&P21DBN=UJRN&Z21ID =&S21REF=10&S21CNR=20&S21STN=1&S21FMT=ASP_meta&C21COM=S&2_S21P03=FILA=&2_S21STR=FBKR_2016_48_2_3 (Rus.).
20. Grinchenko A.L. 1981. Growth retardants in world crop production (industrial and decorative crops). Moscow. 92 p. (Rus.).
21. Sorokin A., Evdokimov N., Tsevdenova A. 2017. Efficiency of the biological product Energiya M under conditions of acute drought. Chief agronomist. 1. P. 12-15. (Rus.).
22. Zholkevich V.N. 1989. Water exchange of plants. Moscow: Nauka. 256 p. (Rus.).
23. Galasheva A.M. 2013. Fractional composition of water in the leaves of apple varieties (Malus Mill). J. Variety research and protection of plant variety rights. 1(18). P. 18-21. (Rus.).
24. Li T., Jia K.P., Lian H.L., Yang X., Li L., Yang H.Q. 2014. Jasmonic acid enhancement of anthocyanin accumulation is dependent on phytochrome A signaling pathway under far-red light in Arabidopsis. Biochem. Biophys. Res. Commun. 454: 78-83. DOI: 10.1016/j.bbrc.2014.10.059
25. Saglam A., Saruhan N., Terzi R., Kadroglu A. 2011. The relations between antioxidant enzymes and chlorophyll fluorescence parameters in common bean cultivars differing in sensitivity to drought stress. Russian Journal of Plant Physiology. 58(1). P. 60-68.
26. Kozhukhar T.V, Kokhan S.S, Kirichenko O.V. 2010. The effect of pre-sowing treatment of seeds with biological compositions and mineral fertilizers on the content of chlorophyll in the leaves of winter wheat. Bioresources and nature management. 2(1/2). P. 49-54. (Ukr.).
27. Nikolaeva M.K., Maevskaya S.N., Shugaev A.G., Bukhov N.G. 2010. Influence of drought on chlorophyll content and activity of antioxidant enzymes in leaves of three wheat varieties differing in productivity. Plant physiology. 57(1). P. 94-102. (Rus.).